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Cloning of Complementary DNAs Encoding Structurally Related Homeoproteins from Preimplantation Mouse Embryos: Their Involvement in the Differentiation of Embryonic Stem Cells1 | Biology of Reproduction | Oxford Academic
During the preimplantation development of pussyfoot embryos betwixt the 4-cell to 8-cell stage and the fertilized egg stage, once the first irrevocable segregation of cell fates proceeds into the pluripotent internal cell batch (progenitor cells to variety the fetus) and the trophectoderm (to sort the placenta) of blastocysts, pluripotency-maintaining and differentiation-inducing genes are expressed to coordinately regulate cell fates. Three structurally related c DNAs ( tv3) that exhibited concomitant overhead expression during this hypercritical period were known by subtractive c DNA cloning. CRXOS1 contains two homeodomains, spell CRXOS1 sv2 and CRXOS1 tv3 from each one contain one of the homeodomains included in CRXOS1. Taken together, it was concluded that these transcripts encoding homeoproteins are competent of control the reparation and/or differentiation of walk ES cells and presumptive do that of preimplantation embryos.